Journal: iScience
Article Title: Identification of novel biomarkers for hypertension and ventricular remodeling based on transcriptomics and machine learning
doi: 10.1016/j.isci.2025.113668
Figure Lengend Snippet: Validate key genes in an HTN-VR mouse model (A–D) (A) Determination of heart rate, systolic blood pressure, diastolic blood pressure, and mean blood pressure ( n = 10); (B) heart weight-to-tibia length ratio ( n = 10); (C) pathological display and quantification of H&E staining and Masson staining in mouse hearts ( n = 10); the black column represents 100 μm; (D) western blot images and relative optical density analysis results of MYH7, ANP, and BNP proteins. β-tubulin was used as the sample control ( n = 9). (E) Detection of mRNA transcription of NR1H2 and MT1E in peripheral blood leukocytes and heart of mouse model using quantitative reverse-transcription PCR (RT-qPCR) ( n = 10). (F–G) ROC regression curve constructed based on the expression of key genes. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Article Snippet: MYH7 antibody , Proteintech Group , 22280-1-AP.
Techniques: Staining, Western Blot, Control, Reverse Transcription, Quantitative RT-PCR, Construct, Expressing